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We found that the immediate promoter regions (M2 and M3) appeared to be unmethylated in all the breast cancer cell lines tested (Figure 1B), though the similar regions have been previously found to be methylated in lung cancer [21].
Previous studies on CUX1 have all focused on genes that contain a CUX1 binding site within the immediate promoter.
Although transcriptional regulatory elements are often found to be concentrated in the immediate promoter region, they are also located in the introns and downstream regions [ 24].
Unlike HCT116 and DLD1 cells, HAND1 in SW480 cells was found to be hypermethylated in a distal M1 region, but not in the immediate promoter region (M2).
Primers were designed to amplify the immediate promoter and exons of OAS1 and RNASEL genes from 13 individual horses by PCR (Table 1).
A 9.5-kb fragment containing the human Cdx2 immediate promoter (Hinoi et al., 2007) followed by the β-globin intron, nlsFlpO, and a bGH-polyA expression cassette was cloned between tandem β-globin insulator element repeats.
Similar(48)
Second, it is known that the main regulatory regions of yeast genes are comprised of their immediate promoters, whereas in more complex organisms the regulatory regions would often lie far away from the gene at unknown locations.
We found that 9 (41%) genes have almost identical sequences in all regions (without deletion/insertions more than 3 bp in length), and we believed that these genes might be regulated by distant trans-regulatory mechanisms other than cis-elements in their immediate promoters.
A BacMam baculovirus was designed in our laboratory to express the reporter protein secreted alkaline phosphatase (SEAP) driven by the immediate early promoter of human cytomegalovirus promoter (CMV).
Insect cell transformation is often accomplished with a DNA construct(s) encoding a foreign protein(s) under the transcriptional control of a baculovirus immediate early promoter, such as the ie1 promoter.
Recombinant adenovirus constructs expressing short and long antisense and sense vascular endothelial growth factor cDNA, under the control of cytomegalovirus major immediate early promoter or the RNA polymerase III promoter, VA1, were constructed.
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