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In addition to its function in the TGN, PKD is thought to play an important role in priming insulin vesicles for transport and immediate fusion (Li et al. 2004; Sumara et al. 2009).
Taken together, it follows that all immediate fusion events started from hemifusion-free point contacts.
Moreover, 'all' (seven) observed traces with immediate fusion upon Ca2+ injection exhibited this behavior.
We found that all instances of immediate fusion events upon Ca2+-triggering started from interacting membranes that were not hemifused.
Specifically, we observed that complexin significantly increased the number of immediate fusion events that occur right upon Ca2+-injection.
Early studies revealed that upon injury a subset of myogenic cells undergo immediate fusion into myofibers without preceding cell division while others, with delayed kinetics, enter mitosis 61.
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44 Patients were randomly assigned to receive either immediate SIJ fusion using iFuse Implant System (n=102) or non-surgical treatment (n=46).
For illustration purposes, only 30 s of the observation period are shown since there was no change in the subsequent 20 s. (C ) Bar graph of the percentage of immediate, delayed fusion, and hemifusion-only events involving SNAREs and synaptotagmin 1.
His immediate and instant fusion of philosophical thought, intimate detail, and the very act of cinematic vision makes this film one of his very greatest, certainly among the singular and crucial movies of recent years.
We discovered a heterogeneous network of immediate and delayed fusion pathways.
DOI: http://dx.doi.org/10.7554/eLife.00109.010 Our experiments revealed the pathway for immediate Ca2+-triggered fusion by synaptic proteins.
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