Phenolic compounds are detected after exposing the plates to ammonia vapours and immediate observation of fluorescent spots under UV light [ 19].
Among the numerous nucleic acid analysis methods of particular interest are those that provide immediate visible or fluorescent response after hybridization to complementary nucleic acid analytes, thus offering easy and instant detection of specific DNA and RNA.
In febrile neutropenic patients with LI, CMV PCR applied on BAL samples has a high negative, but low positive predictive value [ 64], while positive rapid culture, immediate early antigen, direct fluorescent antibody tests, DNA hybridization or cytology from BAL cultures are required to confirm the diagnosis of CMV pneumonia [ 65, 66].
hU6 promoter, human U6 promoter; Pcmv IE, cytomegalovirus immediate-early promoter; GFP, green fluorescent protein.
In addition, fluorescent ligands can provide immediate "real-time" readout of the ligand receptor interaction.
We generated a fusion protein HA-Uprt-mCherry consisting of a vertebrate version of Uprt, a fluorescent protein mCherry for immediate visualization and an HA-epitope for antibody detection [ 13].
The intensity of the localized fluorescent signal in the immediate vicinity of each pore is proportional to the Ca2+ flux through the pore and remains constant as long as the steady flow of ions and ionophores is maintained.
Modulation of fluid flows was observed to effect an immediate, reversible change in mean cell-wide fluorescent intensity.
The currently available transformant rickettsiae expressing fluorescent reporter genes are of immediate practical value by allowing visualization of living rickettsiae in unfixed host tissues as in the case of GFP-fluorescent R. monacensis in Ixodes scapularis [47].
FLI gives us the ability within a fluorescence image of measuring and quantifying dynamic events taking place in the immediate surroundings of fluorophores as well as locating the fluorescent components within the image.
