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The relatively high proportion of tumor cells, despite skipping the usual cell-separation procedures, which would prevent the immediate fixation required to record the authentic ' in vivo' expression, was the consequence of strict patient selection and using of the initial aspiration fraction while the majority of residual normal cells were granulocytes.
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Finally, an ex vivo biomechanical test was completed to investigate the immediate fixation stability of the newly designed screws.
This may be achieved by delayed vascular clamping or immediate fixation following extirpation.
This was followed by immediate fixation or incubation with fresh medium at 26°C for 20 min (chase) to visualize early endocytic compartments with subsequent fixation.
Mice were either removed from the home cage for immediate fixation (no stim) or allowed to explore an enriched environment for 25 min prior to fixation (stim).
Fixation was required until the bar reappeared (see above), the fixation spot disappeared at that moment.
Urgent and immediate resuscitation is required.
If this is observed, immediate action is required.
To avert anoxic encephalopathy, immediate resuscitation is required.
Immediate treatment is required.
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