MCF7 cells treated with heregulin and RWPE cells treated with EGF ligand (or no ligand) were fixed, paraffin imbedded, sectioned and used for antibody validation.
For histopathological studies, lung tissue samples were obtained from mice at 2, 8, and 16 weeks post challenge, fixed in formalin, paraffin imbedded, sectioned and stained with hematoxylin and eosin.
After plants were scored for phenotypes, pWSRi SpPI male inflorescences were fixed, imbedded and thin sectioned.
The fixed tissues were imbedded in paraffin, sectioned at 5 μm, and stained with H & E. All data collected are expressed as mean ± standard error.
Hepatic tissue samples which were 1.5- 2 cm in length or longer, were fixed in 4% neutral formalin, paraffin imbedded, and serially sectioned to stain with Hematoxylin-Eosin (HE), collagen, reticulum fiber and immunohistochemical staining.
Tissue imbedded in OCT was sectioned with a cryostat (Microm HM 550) into 10-mm sections.
Postmortem fundus photographs were taken and the posterior segment was fixed 4 h in 4%PFA, dissected, imbedded in paraffin, and sectioned.
High-purity aluminium rods with 5 mm2 cross-section were imbedded in epoxy resin and corroded from one end.
Myocardial segments from the mid-papillary muscle level were imbedded in the paraffin, sectioned (5µm) and stained with Masson's trichrome and hematoxylin - eosin staining (H&E).
The tumors were imbedded in paraffin and sections were stained with hematoxylin and eosin.
