Sentence examples for imaging yeast from inspiring English sources

Here, we extend our previous studies on imaging yeast cells to image subcellular architecture in human melanoma cells and melanocytes at resolutions as high as ∼6 and ∼20 nm in the directions parallel and perpendicular, respectively, to the direction of ion-beam milling.

For live cell imaging, yeast were incubated in synthetic complete medium at room temperature.

Prior to imaging, yeast cells were grown in synthetic complete media with 2 % glucose (SD) for 24 h with serial dilution to maintain OD below 0.4.

For live imaging, yeast strain GA-1459 was used, which has TEL VIR tagged with four LexA operators (LexO) and an array of Lac operators (LacO), and expresses GFP-LacI to visualize the LacO and GFP-Nup49 to tag the nuclear periphery [ 93].

In this study, we propose a novel strategy to identify drug targets by combining genetic screening and high-content imaging in yeast.

The stoichiometry of QD:hapten used for yeast cell imaging and single-molecule experiments was 1 8.

Our experiments demonstrate that the combination of single-molecule AFM with genetic manipulation is a powerful platform for imaging protein clusters in living yeast cells in relation with function.

Multicolor live imaging of patch components in yeast cells has become an incredibly powerful assay for defining the roles and dynamics of different endocytic proteins and the consequences of mutations or perturbations.

This study uses a combination of co-immunoprecipitation, yeast two-hybrid, imaging and co-sedimentation studies to show that Fab1p, Vac7p, Fig4p and some Atg18p join with Vac14p to form a complex at the vacuole membrane.

All flies were aged for 3 5 days with males and fresh yeast paste prior to imaging.

To study SAC activity in fission yeast, we established a live-cell imaging assay using Plo1 GFP (green fluorescent protein).