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The setup of the AP-SMALDI imaging source is described in detail elsewhere (Koestler et al. 2008).
This method is based on a matrix-assisted laser desorption/ionization (MALDI) imaging source working at atmospheric pressure which is coupled to an orbital trapping mass spectrometer.
Experiments were performed using a home-built atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization (AP-SMALDI) imaging source attached to a linear ion trap/Fourier transform orbital trapping mass spectrometer (LTQ Orbitrap Discovery, Thermo Scientific GmbH, Bremen, Germany).
A FireWire CCD color camera (DFK21AF04) (The Imaging Source, Bremen, Germany) used for imaging, was mounted perpendicular to the side of the sample chamber under observation.
The beam was directed into a microscope (Eclipse T i, Nikon), with a high reflecting mirror for 800 nm as shown, to direct the beam to the objective and split the laser beam from the image for viewing on the CCD camera (DFK41BU02, The Imaging Source).
The specimen is rotated with respect to the imaging source; in this case, electrons and a series of projection images are recorded over a limited angular range (usually −70° to +70°).
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Enter COCAE, a European project to develop a camera capable of imaging sources of nuclear radiation.
Magnetoencephalography (MEG), for example, is an established (usually non-optical) technique for imaging sources of magnetic fields inside the brain.
Multimodal neuroimaging advances the neuroscience research by overcoming the limits of individual imaging modalities and by identifying the associations of findings from different imaging sources.
In short the AOSLO uses a supercontinuum source (Fianium, Inc). to provide both the wavefront sensing beacon and the imaging sources.
Some of the earlier studies using laboratory X-ray imaging sources also demonstrated the brain microvasculature in small animals [ 18, 26].
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