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Simple reflection geometries [ 3] are suitable for imaging skin but cannot image inside the body except during open surgery.
Combined TPF-SHG micrepresentepresent a powerful tool for imaging skin dermis, since the main dermal components, collagen and elastin, can be imaged by SHG and TPF microscopy, respectively [ 4].
But on the screen of MelaFind, a new computer vision system for imaging skin lesions, a jagged blue line shows the actual border of the mole, revealing an irregular lesion roughly the shape of Texas.
This article summarizes the principles and instrumentation of this imaging technique and highlights some of the recent progress in applying it to imaging skin pigmentation and microvasculature under ex vivo or in vivo conditions.
In our particular application for imaging skin cancer, the effects of scattering and aberration must be considered.
Development of noninvasive, high-resolution techniques for anatomically imaging skin cancers or skin related lesions in situ can increase the diagnostic accuracy.
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In a first demonstration, we use the system for imaging human skin at an A-scan rate of 109 kHz.
This is mainly due to difficulties involved in imaging intact skin, the way that different arbors overlap and intermingle, and the relatively large distances that separate the bodies of neuronal cells and the farthest reaches of their arbors.
In clinical settings, at 1064 nm the American National Standards Institute (ANSI) allows for maximum safe laser fluences of 100 mJ/cm compared to 20 mJ/cm at ≤700 nm when imaging through skin [ 18].
In this paper, we perform microcirculation imaging of skin using an extended focus OCT (xf-OCT) system [ 15]. xf-OCT that is based on Bessel beam illumination provides an enhanced depth of focus as compared to standard Gaussian illumination.
Figure 10a,b,c,d shows a breast cyst image with imaging artifacts, a skin lesion image contaminated by texture tissue, a MR heart image with clutter noise and a cell image with multiple objects.
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