Figure 29c shows histograms of the lifetimes of type II and type III bindings that were measured using HS-AFM images taken at an imaging rate of 10 fps.
This rotational motion indicated the association energy to be approximately −1 kB T. The disassembly process of the 2D crystal was also monitored by HS-AFM following chelation of Ca2+ ions. Figure 37 shows HS-AFM images clipped from the successive images (scan range, 500 × 500 nm; imaging rate, 1 fps).
Figure 18a shows two images of D96N bR clipped from successive images at the cytoplasmic surface captured at an imaging rate of 1 fps, under repeated dark illumination cycles.
However, as mentioned above, the AFM's low imaging rate (about 1 min is required to capture an image) has precluded the analysis of the vast majority of dynamic biological processes and therefore limited its usefulness for biological studies.
Single molecule imaging of individual cells was performed by serially scanning the entire cell nucleus through the light sheet at 20 50 ms exposure per 2D image and 300 nm z-steps resulting in a 3D imaging rate of 3 s per volume.
Figure 25a shows successive AFM images of a SERCA molecule in the membrane captured at an imaging rate of 1 fps in the presence of both 10 nM ATP and 100 μM free Ca2+.
Figure 44a shows time-lapse images of the bacterium before and after the addition of lysozyme (imaging rate, 0.05 fps; scan size 5 × 5 μm with 256 × 256 pixels).
There was no significant difference in the head CT imaging rate pre- and post-intervention (39.8% vs. 43.2%, p = 0.358).
The head CT imaging rate for syncope patients was not significantly different during the pre- and post-intervention periods.
The imaging rate is 25 frames per second (FPS), and the average scan speed (va) can attain a value of approximately 2.5 cm/s in experiments.
The imaging rate is 25 frames per second, and the average scan speed reaches a value of approximately 2.5 cm/s.
