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The solid lines represent quadratic fittings, where it can be seen that the velocity profile takes on the shape of a parabola demonstrating a laminar flow mode [ 28]. Figure 6(g) shows a half parabola because only the top half of the cross-section of the blood vessel was imaged due to limited imaging penetration.
The high speed and deep imaging penetration is ideal for Doppler OCT of posterior eye blood vessels.
Imaging penetration using fluorescently labeled active agents also yields accurate lateral and depth spatial information; however, the fluorophore may alter penetration mechanisms.
As a result of an increased scattering length in tissue for longer excitation sources, the imaging penetration depth can be increased significantly using longer excitation wavelengths [ 12, 13].
Our results demonstrated that the PAM system offers much deeper imaging penetration than optical imaging methods, allowing early-stage and sensitive detection of tumor angiogenesis and therapeutic evaluation onset.
Recently, several works have demonstrated the advantages of contrast agents for FIGS in the NIR spectrum, because of its separation from the visible spectrum (white light channel), reduced autofluorescence, and prospects for deeper imaging penetration [ 45, 46].
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Similar results were found when imaging dye penetration into capsule sections or tablets.
While microscopy achieves subcellular spatial resolution, the imaging speed, penetration depth, and field of view are limited.
However, there are no demonstrations of DSI imaging with penetration depth larger than 100um up to date.
Here we have designed and synthesized a fluorescent dansyl-polymyxin lipopeptide probe which retains the native antibacterial activity of the polymyxin B parent structure; and demonstrated its considerable utility for imaging polymyxin penetration and localization in Gram-negative bacterial cells.
Although the achieved sensitivity was sufficient for OCT imaging with deep image penetration, as shown in Fig. 13, the reduction of 10 dB from that expected for the shot-noise limit should be improved.
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