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MR data were acquired using these 2 sequences, with the US transducer connected to the US scanner, which was switched on but had the imaging frozen.
Therefore, many techniques are currently employed to increase the yield as well as accuracy to improve the clinical significance and prognostic value of brain biopsy methods, such as radiological imaging techniques, intraoperative imaging, frozen section analysis, and even robotics [ 1, 2, 4, 8, 10– 16, 17, 17].
For high-resolution, whole-brain fluorescence imaging, frozen brains were embedded using stainless steel Tissue-Tek base molds and Neg-50 embedding medium (Richard-Allan ScienThermo]Thermo]; n = 2 animals) (Pinskiy et al., 2013).
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The polymer melt model evolved from the "liquid" model that Dubochet and colleagues proposed after imaging frozen-hydrated CHO cells with cryo-EM (McDowall et al. 1986).
Tumors were excised on day 7 after the trial, one day after imaging, and frozen in liquid nitrogen fixed in formalin.
For SEM observations, the pretreated CS samples were prepared for imaging by freezing them in liquid nitrogen and then freeze-drying.
The beam damage limit occurring during wet-STEM imaging of fixed cells in liquid thus appears to be comparable with that for TEM imaging of frozen samples.
The formation of nano-bubbles is a known phenomenon from the imaging of frozen samples [20], [21].
Throughout this experiment the US transducer was connected to the US scanner, which was switched on, but the imaging was frozen.
Development of special devices and protocols that would allow easier manipulation and confocal imaging of frozen samples prior to cryo-sectioning, perhaps along the lines as for the conventionally prepared or freeze-substituted material (Pfeiffer et al. 2003; Biel et al. 2003), may be quite useful to assist cryo-sectioning.
After imaging and biodistribution, frozen tissues were stained for Sn using SER-4 followed by anti-rat-biotin then streptavidin-HRP.
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