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In addition, kinetic modeling of dynamic FAZA uptake enables improved inter-tumor normalization achieved through the measurement of the perfusion characteristics of each tumor during the early imaging frames.
Dynamic imaging data were divided into 51 imaging frames (30 × 10 s, 15 × 60 s, 4 × 300 s, 2 × 600 s); 3D imaging data were rebinned into 2D sinograms with a Fourier rebinning algorithm and then reconstructed with 2D filtered back-projection, with a final voxel size of 0.78 × 0.78 × 0.80 mm.
The histogram is best-fitted with a double exponential decay function, with the first decay time constant τ1 = 12.3 imaging frames (24.6 sec) accounting for 97% of the molecules.
It is sensitive to outliers, and therefore, only sensitive to sudden, drastic changes of intensity between imaging frames.
To reduce photobleaching, the laser was turned off automatically between imaging frames at frame rates of less than 10 Hz.
Ten minutes after injection of D-Luciferin, mice were imaged using an in vivo imaging system (IVIS) Lumina (Xenogen, Almeda, CA, USA) camera by taking consecutive 10-s to 2-min imaging frames.
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Several experimental systems show a drift: a slow oriented motion of the sample versus the imaging frame, which is caused by various factors of the experimenting apparatus.
In the experiments with Numberpixel=127,000, many test cases for various M and L combinations achieved real-time video imaging frame rate for medical ultrasonic imaging, which was higher than 20 fps.
Synchrony of Ca2+ traces of a pair of astrocytes was calculated using the Fisher's exact test in which traces of fluorescence intensity of Ca2+ indicator was divided into two periods, i.e. periods with or without Ca2+ surge, for each imaging frame.
However, direct 3D+T imaging frame rates remain insufficient for cardiodynamic analysis.
Each imaging frame consisted of 500 x 200 pixels (axial x lateral).
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