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In the experiments with Numberpixel=127,000, many test cases for various M and L combinations achieved real-time video imaging frame rate for medical ultrasonic imaging, which was higher than 20 fps.
The individual blood cells can be clearly observed using a fast (30 Hz) imaging frame rate [ 14].
The OCT imaging frame rate of this instrument is 20 to 50 frames/sec (with 1024 or 512 A-lines/frame), while the fluorescence imaging frame rate is 5 frames /sec (200 ms integration time is needed to provide good SNR in the fluorescence mode).
After 10 s of imaging (frame rate of 100 ms), the flow chamber was perfused with buffer supplemented with 5 mM ATP and 800 nM Lis1 as indicated.
Fiber-based, video-rate fluorescence DOT could additionally improve the flexibility of imaging by adapting to varying tissue curvatures and performing simultaneous multiple point illumination and collection, thus increasing the imaging frame rate.
The micromotor could rotate uniformly with a driving voltage less than 5V at a speed from 1,200 rpm to 72,000 rpm, corresponding to an imaging frame rate from 20 fps to 1,200 fps.
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One important advantage of flood-illumination methods is that areal imaging frame rates can be an order of magnitude faster compared to scanning modalities (e.g. the AOSLO typically ranges from 30 to 60 Hz [ 18, 19]), owing to the development of sCMOS technology that can acquire en-face images at up to 1000 Hz or more depending on the field-of-view required [ 20– 20].
However, direct 3D+T imaging frame rates remain insufficient for cardiodynamic analysis.
This technique was validated in a canine model with intermittent imaging with destructive frames at increasing imaging frame rates, microbubbles being continuously infused by means of a syringe pump.
Presently photoacoustic imaging frame-rates are limited by the 10-Hz laser pulse-repetition rates and the requirement for one laser-pulse-per photoacoustic A-scan line.
Because the early diastolic filling period is short (approximately 100 ms), accurate measurement of early diastolic velocity/function requires high temporal resolution of the imaging technique (frame rate >100 frames/s), which is provided by pulsed tissue Doppler and in high-quality color Doppler systems, but not with MRI [ 27].
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