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Until then, evidence is needed to support a reappraisal of role of surgery in these injuries, especially if outcomes are improved through advances in surgical approach, intra-operative imaging, fixation technique, and familiarity in the hands of "experts".
The length of the fracture-free period to ascertain incident cases had a variable effect on over-ascertainment across fracture sites, as did the use of imaging, fixation, or repair service codes.
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FLIM imaging preceded fixation with formaldehyde.
Post-imaging fixation, staining and paraffin sectioning followed normal protocols [34].
Afterward, the OptiMEM was replaced with complete growth media for 15 min at 37 °C before fixation, imaging, or harvesting virus.
Because IV-administered antibodies were at a significantly lower concentration than endogenous IgG, it is likely that this discrepancy represents the limited sensitivity of IHC staining techniques, as well as tissue fixation, fluorescence quenching, imaging parameters, and image analysis.
18 24 hrs after plating passaging medium was changed to complete culture medium and cells were grown for either 48 or 24 hrs prior to live cell imaging or fixation and immunocytochemistry procedures respectively.
Open image in new window Fig. 5 Preoperative AP X-rays (a) of 10-year-old female patient who sustained subtrochanteric fractures, intraoperative imaging after fixation (b).
Certain fluorescence labels may influence biochemical processes in cells or cannot enter live cells to access cytoplasmic molecules (often fluorescence imaging requires fixation of the cells).
Confocal imaging after fixation confirmed that the fluorescent particles were contained within the cells (data not shown).
Indeed, differences increased when the time interval between live cell imaging and fixation was prolonged.
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