Apparent temperature and image contrast were demonstrated by imaging experiments.
Modern imaging experiments, with ever increasing scale, rule out manual/visual image processing and analysis.
TM performed the calcium imaging experiments and analyzed the data.
designed and performed the biochemistry and imaging experiments and participated in electrophysiological experiments; M.M.P. designed and performed agonist- and stimulation-evoked electrophysiology and participated in imaging experiments.
R.M.M., J.D. and R.B.P. performed and analyzed the initial calcium imaging experiments and A.G., N.D.-I.
For all calcium imaging experiments, Fluo-4AM (Invitrogen) was mixed with 10 μl Powerload (Invitrogen) and vortexed for 15 s.
For example, imaging experiments carried out on blind people show that when they learn to read Braille, "visual" areas of their brains light up.
During imaging experiments, animals are mostly immobilized by light anaesthesia.
Pilot imaging experiments, however, showed this was not the case.
Tasks will involve advanced optical engineering, signal processing, and live animal imaging experiments.
Further investigation of this awaits imaging experiments using cytoskeletal markers.
