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This chapter presents an overview of the complex and ever-developing landscape of imaging cytometry, highlighting the imaging and quantitative performance of a wide range of available instruments based on their methods of sample illumination and the detection technologies they employ.
These cells grow attached and their fluorescence intensity was measured by imaging cytometry (laser scanning cytometer; LSC) [ 104].
To achieve this, we use imaging cytometry to provide spatial information on the localization of quantum dots within cell vesicles.
Overall, this on-chip technique is equivalent to imaging cytometry as it modulates and accordingly changes the diffraction patterns of different cells that are specifically labeled with particles.
The differences in intratumoural viral titres after PDT treatments were also reflected in numbers of EGFP+ cells measured by imaging cytometry on single cell suspensions.
As NOX2 activity requires the fusion of the membrane-bound p22 and cytosolic p47 components, we examined the co-localization of p22 and p47 in human CD11b+ PBMCs by imaging cytometry to confirm our FLIM data with an independent approach.
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Microscopic analysis of cells immobilized after sorting by flow cytometry, imaging flow cytometry [16], and combination of flow cytometry with cell fractionation [17] are potential alternatives to hiMAC in particular for simple readouts such as overall protein levels.
Different approaches have been utilized to experimentally quantify the cellular or intracellular dose of NPs, such as atomic emission spectrometry [ 23], laser ablation ICP-MS [ 24], flow cytometry, imaging flow cytometry, and electron microscopy [ 25– 28].
In an effort to garner the most information from limited numbers of human cells, we designed a quantitative method to study NK cell function using imaging flow cytometry (IFC), which combines multiparametric flow cytometry and fluorescence microscopy.
This ranged from a 1.65-fold increased level of expression in clone 3 to a 2.25-fold elevated expression in clone 5. Representative images of artemis staining of cells derived from imaging flow cytometry is shown in Figure 7B.
Since flow cytometry only gives data regarding total shifts in fluorescence of a population, imaging flow cytometry and live imaging microscopy studies were performed to determine if the NLS augmented nuclear accumulation of the peptides.
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