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After staining, fluorescence images were taken using a QICAM Fast Cooled Mono 12-bit camera (Q Imaging) attached to an Olympus microscope with Capture 2.90 (Olympus, Center Valley, PA).
For confocal imaging fast Nipkow disc-based confocal imaging attached to a high sensitivity (iXon Andor) CCD camera, was used to enable acquisition of images at 60 200 fps and thereby accurate measurement of temporal and spatial characteristics of Ca2+ signalling in the muscle cell bundles.
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Confocal images were taken using a Leica SP5 confocal imaging system attached to a Leica DMI 6000 inverted microscope with a motorised XYZ stage for multiple-site imaging.
For cell adhesion assay [25] and time lapse imaging of attached keratinocyte, equal number of keratinocytes from knockout and control mice were plated per well of ColI/FN coated 12-well tissue culture plates.
The hardware consists of a handheld imaging probe attached to a laptop computer.
Images were taken using a Leica (Europe) AOBS SP2 confocal imaging system attached to a Leica DM IRE2 inverted epifluorescence microscope.
Experiments were performed using a home-built atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization (AP-SMALDI) imaging source attached to a linear ion trap/Fourier transform orbital trapping mass spectrometer (LTQ Orbitrap Discovery, Thermo Scientific GmbH, Bremen, Germany).
A Leica TCS SP2 (Leica Microsystems, Bannockburn, IL) confocal imaging system attached to a Leica DME 14 upright microscope equipped with Ar/Kr lasers were used to study BY-2 cells treated with CPNs and FM4-64 staining protoplasts.
The imaging head is attached to a flexible gooseneck arm, which permits positioning of the imaging head at extreme angles virtually anywhere over the surgical field.
To take advantage of the computer controlled microscope functions the OPS imaging probe was attached to the shaft of the microscope.
In folate targeting strategies [ 6– 8, 14– 16], the 'cargo', which is a drug or imaging agent, is attached to folic acid via a linker and together this system is known as a folate conjugate.
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