Exact(2)
Plasma membrane-localized CD147 protein expression was quantified in patient samples using immunohistochemistry and multispectral imaging, and expression was compared to clinico-pathological features (pathologic stage, Gleason score, tumor volume, preoperative PSA, lymph node status, surgical margins, biochemical recurrence status).
By generating transgenic mice with L cell-specific expression of a fluorescent protein, we studied the characteristics of primary L cells by electrophysiology, fluorescence calcium imaging, and expression analysis and show that single L cells are electrically excitable and glucose responsive.
Similar(58)
For live cell imaging and protein expression, COS1 cells were transfected with Fugene and with LipofectAMINE respectively, according to the manufacturers' instructions.
MF acquired and analyzed imaging and gene expression data.
A similar relationship between high-content imaging and gene expression data was observed for inhibition of rHB growth.
Overall, high-content imaging and gene expression data supported that activation of AHR has a stimulatory effect on growth of rHpSCs and loss of viability of rHBs.
A similar relationship between high content imaging and gene expression data for TCDD was observed for Afp, Alb, and Sox17 (Fig. 7E).
We have demonstrated a simple and effective method for isolating live single cells for microscopic imaging and gene expression analysis by RT-qPCR.
Finally, molecular imaging and gene/protein expression studies will be explored in conjunction with MRI to interrogate the molecular mechanisms involved in cancer initiation and progression.
The combination of imaging and gene expression, referred to as "radiogenomics," has the potential to give insight into tumour biology that would be difficult to acquire from either technique alone.
All patients have been diagnosed with OSCC and have reached remission, after successful therapeutic intervention (i.e. surgery, chemo/radio-therapy). Thereafter, we collect clinical, imaging and gene expression data, both from the primary tumor as well as from circulating blood cells, at the baseline state of the patient.
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