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Overall, both Raman imaging analysing methods (k-means clustering and ANN) were capable of visualization the cell morphology.
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To determine whether Trak1 colocalizes with mitofusins in these mitochondrial subdomains, we performed dual-color 3D-SIM super-resolution imaging analyses to compare the spatial distribution of endogenous Trak1 with that of Myc-tagged Mfn1 or Mfn2 on mitochondria.
To further characterize Trak1 depletion-induced mitochondrial morphological phenotype, we performed super-resolution imaging analyses using three-dimensional structured illumination microscopy (Huang et al., 2009; Fallaize et al., 2015).
Subsequent time-lapse imaging analyses reveal that inhibition of microtubule-mediated cellular processes, including cell migration and mitotic chromosome alignment, is the earliest events affected by Celastrol.
Our in vivo imaging analyses reveal that the matching of a neuron with hair cells of identical orientation is evident only when the innervation process is observed directly at high temporal-resolution over long periods.
The PER olfactory conditioning protocol allows for the precise control of stimulation parameters in behavioral studies as well as for simultaneous linkage to neurophysiological and imaging analyses of brain activity [30] [33].
The ideal platforms for biomarker discovery include genomic, transcriptomic, proteomic, metabonomic and imaging analyses.
Intriguingly, imaging analyses from this study support the observed reciprocal changes in membrane cholesterol and F-actin.
VU performed histological and imaging analyses, and drafted portions of the manuscript.
In vivo imaging analyses of the transplanted cells confirmed these results.
Excreted proteins were evaluated with the assistance of proteomics using two-dimensional analyses followed by imaging analyses.
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