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Animal orientation and tumor position should be kept constant in the bowl during experiments to make sure that each scan was in the same position in favor of comparison and imaging alignment.
There was no relation between the alignment percentage and tumor response (Figure 5), signal to noise ratio of either the liver or normal parts of the lung, or with the distribution of the original SUV. Figure 5 Relation between thoracic imaging alignment and tumor response according to RECIST criteria per patient.
Our imaging, alignment and tracking methodology, which allows a large cell population spanning these different domains to be followed, will permit such comprehensive analyses.
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To remove motion artifacts from in vivo calcium imaging, the ImageJ plugin TurboReg for image alignment (Thévenaz et al., 1998) was used.
The aim of our study was to evaluate the association between patellar alignment by using magnetic resonance imaging images and radiographic manifestations of patello-femoral osteoarthritis (OA).
The resulting workflow for 3D DESI-imaging MS dataset compilation was devised based on image alignment and peak matching algorithms published previously [ 23].
The imaging system is built on a customised trinocular (three channel) rigid endoscope (Intuitive Surgical, Inc., Sunnyvale, USA) with computer vision techniques developed for image alignment.
However, due to imaging noise, mis-alignment between multiple channels and many other imaging problems, there is always a classification error, leading to wrong detection of chromosomal abnormalities.
Specifically, the gradient domain approach has been successfully applied to high dynamic range (HDR) imaging [1], image stitching [2 5], filtering [6], alignment [7], matching [8], etc.
Therefore, as explained in the Materials and Methods and Text S1, we used several procedures to ensure that the microscopy imaging and alignment were stable over the 3 4 hr period of luminescence observations and that any observed heterogeneity in the light output from individual cells was not a detection artifact.
On each day of imaging, camera alignment was performed using 0.5 µm diameter fluorescent beads.
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