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Our functional imaging run consisted of 200 whole-brain functional T2*-weighted echoplanar images (slice thickness, 3 mm;.75 mm skip; 32 axial slices; repetition time, 2000 msec; echo time, 30 msec; flip angle, 90°; matrix, 64 × 64; field of view, 192 mm; interleaved slice acquisition).
Analysis of these images, slice by slice, is cumbersome and inefficient.
Hence, laminae borders were extrapolated from analysis of dark-field images (slice morphology), ChAT and NeuN immunoreactivity patterns, and knowledge of the adult rat spinal cord.
Those related to physiological processes can be considered static or dynamic in relation to the temporal scale of a 2D acquisition and will play out on different spatial scales as well: globally across the entire images slice, and locally depending on the constituents and their relative fractions inside the MRI voxel.
Configuration of a cylindrical phantom (A) and summed transaxial images (slice thickness, 14 cm) at 84 keV with MEGP (B) and HEGP (C) collimators.
Axial, coronal, and sagittal images (slice thickness 2.5 mm, slice oversampling 15%) were used.
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Each scan took approximately 30 min and created 381 micro-CT images slices.
Three-dimensional stacks of images were acquired with a 780 Laser Scanning Confocal microscope (Zeiss) using a 20× (1 μm image slice), 40× (0.6 μm image slice), or 63× (0.2 μm image slice) objective to observe the entirety of the CeA.
The present article aimed at constructing a canonical geometry of the human vocal fold (VF) from subject-specific image slice data.
(a) Original MR image (slice 80).
Phase-shifted images required approximately 0.02 s/2D image slice to generate.
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