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Negative controls were carried out using only the secondary antibody omitting the primary antibody (200 μL of goat anti-rat IgG AlexaFluor 488 diluted 1: 600 in PBS), where sections were incubated for 2 h in the dark before imaging, results were negative (images not shown).
Figure 4 shows the evolution of activation patterns for each protocol, in time-averaged 5 s blocks for the first 30 s for Subject 1. Similar patterns were found for the other two subjects (time-series images not shown) and were repeatable between the two imaging sessions for all subjects.
In film and video images, we see body after body fall from the towers and tumble toward the ground, horrifying images not shown on television then; even now, we do not see the bodies as they land or after.
AFM images were taken at several resolutions (images not shown).
Both the superior and inferior costo-transverse ligaments are recognizable on adjacent MR images (not shown).
No spheres were observed to penetrate the porous matrix in cross-sectional images (not shown).
Facet joint subluxation was seen on the parasagittal images (not shown).
There was also pericardial thickening in black blood images (not shown here).
The calcification of the bones was severely diminished, resulting in non-diagnosable CT images (not shown).
There was no diffusion restriction or contrast enhancement (images not shown).
Images show 3D reconstructions of AFM height images (not shown) with the corresponding deflection images.
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