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The mean cross-sectional fibre areas obtained from each image were analysed by dividing them into 12 equal fields and choosing one by random in which at least 150 fibres were measured through the use of image analysis software (Image-pro plus; MediaCybernetics, Bethesda, USA).
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The scanned image was analysed with the GenePix Pro 3.0 software (Axon Instruments, Foster City, CA, USA).
Each image was analysed using the ImageJ software (http://rsbweb.nih.gov/ij/).
The acquired image was analysed using ImaGene 3.0 software (Bio Discovery, Los Angeles, USA).
The signal enhancement profile of every voxel in every image was analysed individually using iterative optimisation.
Images were analysed using the Maestro spectral imaging software as previously described (Kampmeier et al, 2010).
Images were analysed using a GE Xeleris functional imaging workstation (GE Healthcare, Hatfield, United Kingdom).
Images were analysed using Fiji (ImageJ).
Images were analysed using Image J to assess cell migration.
Images were analysed using ICY image analysis software55.
Images were analysed with Living Image v4.4 software.
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