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Reconstruction of the 2D and 3D PA image was performed using Osirix imaging software (OsiriX Foundation, Geneva, Switzerland).
The quantitative analysis image was performed using the imaging software Image J. The relative distribution of p27kip1 protein between the two compartments nucleo/cytoplasm, was calculated by measuring the pixel average signal both in the nucleus, cytoplasm and nucleo/cytoplasm compartments.
For each specimen calibration of the image was performed in the first frame.
In this study, an accuracy assessment of the classified year 2011 image was performed on the basis of 117 ground samples out of 299 plots.
The fusion between the 2D isochronal activation map and the fluoroscopic image was performed in a 6-step process (see Figure 12), as outlined in the following.
b Conventional image was performed to evaluate the extensiveness, showing complete involvement of the tibia, with fracture lines in the proximal and distal metaphysis, and mid diaphysis.
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Reconstructions from a test image were performed.
A supervised study, comparing some areas with evidence of biological crusts with the data shown in a RGB ortho-image, was performed by a human expert and was considered to be ground.
Imaging was performed before PNF resection.
The coregistration of images was performed with sub-pixel accuracy.
Imaging was performed during breath-hold at end-inspiration.
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