To study the structural integrity of individual insulin granules during exocytosis, we imaged uptake of the fluid-phase tracer sulforhodamine-B (SRB).
As evidenced from the γ-scintigraphic images, uptake of the folate-99mTc complex (EC20) in spleen, liver, kidneys, and limbs is substantial in non-immunized rats treated with folate-FITC, but largely limited to the kidneys (which ubiquitously express the FR on the apical surface of their proximal tubules [ 31]) in KLH-FITC-immunized rats treated with folate-FITC (FTI).
Extensive efforts have been made to specifically image glucose uptake, such as with positron emission tomography, magnetic resonance imaging, and fluorescence microscopy, but all have limitations.
This data demonstrates the ability of TKI-PET to image TKI uptake in these tumors in an effective manner.
According to the bone scan image, hot uptake was found in the anterior mandible which resembled bone metastasis.
After initiation of the therapy (bottom image), tracer uptake resolved, while the size of the metastases did not change.
Vascular activity was clearly visible on the final summed image because uptake in the brain regions was negligible.
The most recent implementation of the algorithm, 3-FLAB, fits the image voxel uptake distribution into three classes: background, tumor, and tumor subvolume[37].
In addition, fluorescence microscope (IX73, Olympus, Japan) with 488 nm excitation and 510 550 nm emission was used to image the uptake of nrGO/DOX by A549 cells.
Here, we aim to image tumor uptake of APOMAB® (DAB4) and to define its biological correlates.
