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Unlike in conventional optical imaging, the imaged antibodies are close to the size of the resolution used to image them (monoclonal IgG are between 5 and 20 nm depending on axis) (Harris et al., 1995), and so steric crowding becomes an issue when double labeling very small structures.
Then you image them and you find out some circuits are still relatively preserved, while most of the rest of the brain is not".
Rather, they're simply a trick of the eye abetted by the peculiarities of the powerful microscopes used to image them.
"We've looked at systems like cardiac myocytes, [and] been able to image them between the 'beats' of the cells," Cox says.
However, some infraclinic breast tumors are not accessible by conventional guidance due to excessive depth inside the breast, their small size or technical inability to image them by mammography or ultrasonography.
But it has proven difficult to purify and image them; so far, the best researchers had produced was a fuzzy electron-microscopy picture of what looked like a flat string of beads.
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They stuck five different kinds of molecules on a copper surface and imaged them; none, however, would sit still.
For each condition, we selected individual cells and imaged them at 3, 4 and 6 dpf (Fig. 2Ba i).
On each day, we reacted several embryos with DIFO-488 and imaged them by confocal microscopy.
We stained gels with ethidium bromide and imaged them using a Computar H6Z0812 camera with FOTO/Analyst PC Image software.
Then, at several stages of development, we reacted the embryos with an Alexa Fluor 488 conjugate of DIFO (DIFO-488) and imaged them by confocal microscopy.
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