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The radius of Petzval image surface is not equal to the focal length of the primary mirror or the focal length of the system.
We propose a new method to estimate image bias field by an intuitive assumption that the overall trend of image surface is associated with bias field and the true bias field can evolve from image surface.
In this model, an image surface is considered as a three-dimensional terrain which is composed of valleys and mountains.
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Counting the number of intersections of capillaries with arbitrary grid lines and measurement of total capillary length relative to image surface are similarly reliable measures of FCD [ 14].
The entire picture surface was imaged in six recordings of 4096 × 4096 pixels each.
To obtain immunofluorescence images, surfaces were incubated for 2 h with 10 μg/mL fluorescein-conjugated mouse anti-human GPIbα IgG antibody (clone HIP1, BD, Germany) in PBS to label GPIbα and verify biomolecule conjugation to the Au-NPs.
SEM image of interlaced surface is shown in Figure 1c.
The topographical image of the surface is obtained together with the local size of photocurrent simultaneously.
On these root images root surface was determined using the image analysis software ImageJ (vers. 1.40 g, National Institutes of Health USA, http://rsb.info.nih.gov/ij/java1.5.0_16).
The SEM image indicates that the surface is relatively smooth and contains many pores.
The SEM image of the etched surface is shown in Fig. 8.
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