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Incubation with FITC-labelled anti-rabbit and TRITC-labelled anti-goat IgG secondary antibodies (Santa Cruz Biotechnology) was performed for additional 30 min. The subcellular localization was examined by CLSM (Leica DMIRRE, Wetzlar, Germany) and analysed with Image Span software (Leica TCS-NT).
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The width of the image spans about 14 cm.
The faint red halo that can be seen across much of the infrared image spans several light-years, the researchers estimate.
Whereas the Milky Way's greatest star clusters radiate millions of times more light than the sun, Segue 3, shown here in an image spanning 64 light-years, ekes out a mere 90 suns' worth of light.
Defying the moment of creation, the diverse image spans instead the life of the fruit, commenting on its inevitable decay.
The slides of this tumor (beginning with the section represented by the top left image and ending with the section represented by the bottom right image) spanned 48 sections (4 μm each).
The images span eight decades, and include iconic and previously unseen images ranging from his early street photography of the American South and snapshots of Europe after World War II, to editorial and advertising photography for Vogue, to pictures of still life, self-portraits, and well-known portraits of Truman Capote and Langston Hughes.
The images span a time period of just over 25 minutes, from 16 39 03 to 17:05:39 Universal Time (UT).
While this is not the first in vivo demonstration of speckle images at multiple exposure durations [ 17], we note that these images span almost 3 orders of magnitude of exposure duration which is possible with an inexpensive camera using the MESI approach.
Multiplane Z-series time-lapse images spanning the dimensions of imaged microglia were collected at a 512×512 pixel resolution at a rate of one image stack every 10 seconds.
Photographers Max Dupain and David Moore produced enduring images spanning portraiture, cityscapes, the suburbs, and the country.
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