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Indeed, for familiar image sessions (n = 23), we found that, as expected, the orientation discrimination threshold was <5o in block 1 and performance did not improve further during the session (p > 0.5 for each block comparison; ANOVA test, Figure 6A inset).
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We used Build and ArcBuild [26] predefined XNAT tools for image session scan selection and running processing steps, respectively.
In subsequent imaging sessions, imaged regions were re-localized based on the unique pattern of blood vessels.
Imaging sessions for the second patient (B) show a clear discrepancy between pre-therapeutic (left) and post-therapeutic (right) imaging sessions.
Imaging sessions were generally 1 h long, with 30-sec time intervals.
Imaging sessions were performed in one visit, which required approximately 30 minutes per subject.
Imaging sessions had to be very short (1-2 minutes) as the fish had to be completely paralyzed.
Imaging sessions lasted for 45 min; z-stacks were taken with 2-μm step size every 3 min for the duration of the imaging session.
Imaging sessions began approximately 1 2 h after initial anesthesia induction, and rats were maintained on 2% isoflurane for the scan duration.
In subsequent imaging sessions, previously imaged volumes were identified by eye using the unique blood vessel pattern and fine adjusted by the positions of preexisting plaques.
In subsequent imaging sessions, previously imaged volumes were identified by eye, based on the positions of blood vessels, neuronal cell bodies and processes, as well as plaques.
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