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Electron Microscopy and Image Processing Specimens for electron cryomicroscopy were prepared by the back-injection method in 4% trehalose (Breyton et al., 2002 ).
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Beside direct deflection measurement at some discrete points within the specimen domain, image processing technique is also used to obtain complete deflection profiles under loaded conditions.
In this study we have investigated OCT imaging of human glioma biopsy specimens and present a first report of a clinical pilot evaluation of intraoperative time domain OCT imaging and post-acquisition image processing as a new intraoperative imaging modality.
The advantages and limitations of each technique are illustrated by their application to determining the 3D ultrastructure of human blood platelets, by considering specimen geometry, specimen preparation, beam damage and image processing methods.
Digital images were obtained using a Leica SCN400 system, and the H&E or immunofluorescently stained specimens were analyzed using IP Lab image processing software, as described earlier 30.
The results indicated that the discontinuity of mesoscopic structures in asphalt mixture was fully considered in digital specimen based on the digital image processing.
CH took the image stacks of the specimens and was also involved in image processing.
Correlations between compressive strength values of the obtained concrete specimens and the results obtained at the end of image processing were sought.
By exploiting strategies in real-time serial communication and image processing, the prototype acquires uniformly dense point cloud from a geometric specimen.
The tissue sectioning, specimen preparation, in situ hybridisation, fluorescence microscopy and image processing have all been described previously (Prieto et al. 2007).
As a result, CTF correction is now widely incorporated in various image processing software packages, and routinely performed for structure determination of vitrified biological specimen.
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