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All images were processed with the MRC image processing programmes to correct for crystal lattice distortion (Henderson et al., 1986).
Image processing was performed using the MRC image processing programmes.
To determine the lattice parameters, a projection image of a single-layered 2D crystal was calculated from the corresponding z-slices of the tomographic volume by first extraction and conversion into a projection image using the IMOD package (Kremer et al., 1996) and then processing the projection images using the MRC image processing programmes (Crowther et al., 1996).
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An automated sizing and counting approach based on Image J (a Java-based image processing programme developed at the National Institutes of Health) [1] has been performed for all near-bulk irradiation data.
Image analysis was performed using Image J software (a freely available Java image processing programme).
A fully automated method, which attempts to mimic Cumulus, was recently developed by some of us (JL, KC, KH and PH) based on a public domain Java image processing programme, ImageJ (Li et al, 2012) (referred to as ImageJ-based method hereafter).
The density of protein bands was determined using ImageJ, a public domain Java image-processing programme.
The standard image processing package (ImageJ, public domain programme developed at the Research Services Branch of the National Institutes of Health, USA) was used to carry out the ESEM image analysis [39].
With the focus on image processing, 16GB of DDR3 1333Mhz Ram from Kingston seemed just the ticket.
The ace up this machine's sleeve is its Darbee image processing.
But advanced image processing that provides rich detail isn't always the best option.
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