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All subjects using a device started with a sweep rate of one image per two seconds, but were free to accelerate the sweep rate to one image/s after one to four series.
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A temporal resolution was reached of one image per four heartbeats.
At least six images per section, four sections per sample, and three samples per group were included to obtain the calculation.
In both cases, 3D-SIM image stacks were acquired with a z-distance of 125 nm and with 15 raw images per plane (five phases, three angles).
A piezo Z stage sweeps the objective axially to record a 100 μm target brain volume with closed loop real-time Z tracking (2 μm axial step per DIFF image, 50 DIFF-sectioned images per sweep, two sweeps per second).
Counts were performed on a total of 12 contiguous, non-overlapping images from each of three coverslips (a total of 36 images per experiment, six experimental repeats).
At least six pores per image, three images per sample, and three samples per group were included to perform the calculation.
To determine the yield of myelinated axons produced by the culture method, the numbers of myelinated axons crossing superimposed grid lines were counted for 13 experimental repeats (20+ images per coverslip, three coverslips per experiments, 13 experiments).
The capsule takes two images per second during eight hours, depending on battery power.
(B) The uptake of beads per spleen area was quantified using ImageJ software in six to ten confocal images per mouse with six mice per group after injection of fluorescent beads.
Rastor imaging (five images per specimen) was conducted on four specimens (n = 20) utilizing a fluorescent microscope (Nikon Eclipse TE2000-S), and cells were manually counted to quantify viability.
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