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Movies were acquired as one image per 2 seconds over 60 to 150 frames, and are presented at playback rates of 15 frames/second.
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The camera is set to capture one cloud image per 15 s.
The system takes this information as input and through an HTTP (Hyper Text Transfer Protocol) request, Google Street View image are queried at the spatial frequency of one image per 10 m via Google Maps static API.
The total image acquisition time was 1.3 seconds for a given depth, and images were acquired at the rate of one image per 1.9 seconds.
The factorial study design was based on 30 subjects (3 images per subject), 10 observers (3 readings per observer).
The 316 images (79 images per section, 4 sections) with 5-mm reconstruction thickness generated by CT perfusion were transmitted to AW4.1 workstation via a local area network.
The values from three images per Robo2 variant were averaged; error bars represent the standard error of the mean.
The values from three images per Robo2 variant (Robo2, Robo2ΔIg1, Robo2ΔIg2, Robo2ΔIg1+2) were averaged; all images were acquired using identical imaging parameters.
Exposure times were 20 ms (340 nm) and 5 ms (380 nm), and the rate for [Ca2+] measurement was set to one pair of images per 1 sec.
The temperature data are presented as the mean for 30 min sampling periods, that is six images per 30 min period.
In practice, 24 embryos for one condition were imaged per 384-well plate, but experiments were repeated at least in triplicate such that, on average, 50 larvae were analyzed per condition (see below).
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