Histograms of total DNA content as determined by flow cytometry, nuclear DNA content as measured manually from 4', 6-diamidino-2-phenylindole (DAPI) images (manual) and nuclear DNA content as measured automatically from color-deconvolved images (auto) were fitted to a model of pre-S-phase, post-S-phase and during S-phase changes in DNA content through the cell cycle.
If you wish to segment this tumor manually, you may experiment with thresholding tools to select the soft tissue HU range best depicting the tumor alone to define a starting ROI within the left upper thorax, followed by image-by-image manual edits to segment the tumor.
Because of the high contrast of the InAs QDs in the HAADF-STEM images, manual segmentation of the tomogram was carried out in order to locate the QDs.
We additionally found that the implemented thresholding frequently required per-image manual adjustment, making the automated, rapid analysis of leaves more time consuming.
In contrast to emphysema, which can already be quantified automatically in CT images, manual interaction is often required for analysis of CT images with opacifications.
Our results support the notion that manual cell counting is time consuming (47 images; manual counting time = 2.4 hours versus automated computing time = 14.5 seconds) and subject to operator bias (hence our experimenter was blinded).
On the stack of the reconstructed micro-CT cross-section images, manual regions of interest (ROI) of an irregular anatomical contour were drawn in the subchondral trabecular bone region for the medial and lateral tibial plateau, for both the MIA-injected knee and the control knee (software CT Analyser, V 1.8.05, Skyscan, Kontich, Belgium).
Automatic detection methods are faster and easier to apply to multiple images than manual image interpretation.
Header image of Manual for iPhone shot with Manual for iPhone.
d, Composite image comparing manual (annotation) and automatic (prediction) segmentation.
