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Following incubation with the appropriate horseradish peroxidase- (HRP-) conjugated secondary antibodies (Jackson ImmunoResearch), protein bands were visualized using enhanced chemiluminescence (ECL) plus western blotting detection reagents and exposed in a Bio Image Intelligent Quantifier 1D.
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His descriptions of Putin and Assad were not studies of individuals but projections of his self-image: intelligent, strong, and pugnaciously — and successfully — opposed to Barack Obama and Hillary Clinton.
Slides were allowed to rest for 60 min in the dark and stored at 4°C if necessary before visualizing under an Olympus IX81 fluorescent microscope (Carson Scientific Imaging Group; Ontario, Canada) using Slidebook 2D, 3D Timelapse Imaging Software (Intelligent Imaging Innovations Inc.; CO, U.S.A).
Measurements were taken by using the SlideBook Imaging Software (Intelligent Imaging Innovations, Inc., Denver, CO).
Then, the cells were directly imaged with Intelligent Bio-image navigation FSX100 (Olympus Optical Co., Ltd, Tokyo, Japan).
Images were acquired with a CCD camera using Slidebook imaging software (Intelligent Imaging Innovations).
The Company's major products include digital monitoring systems, network monitoring video recorders, image servers, intelligent analyzing software and central monitoring software, among others.
Stereological analysis of the 2× montage was performed by masking the hepatic area and placing 500 um×500 um regions with spacings of 1000 um×1000 um and a random offset of 69.4 (x-coordinate) and 513.4 (y-coordinate), on the image (SlideBook, Intelligent Imaging Innovations, Denver, CO).
Images were acquired on an Axiovert 200M confocal microscope (Zeiss, Peabody, MA) with a xenon arc lamp in a Lambda DG-4 wavelength switcher (Sutter Instrument), and were processed with Slidebook imaging software (Intelligent Imaging, Denver, CO).
They were then imaged using an Intelligent Imaging Innovations (3i) Marianas system, which incorporates a Zeiss Axiovert microscope, CoolSnap CCD, and Slidebook software.
The specimens were examined with a Zeiss Axiophot fluorescence microscope equipped with a charge-coupled-device camera controlled by the SlideBook deconvolution image-processing software (Intelligent Imaging Innovations, Denver, CO).
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