The intensity of the spot was measured using phosphor imaging with image gauge software.
Antibodies were detected using horseradish peroxidase conjugated IgG secondary antibody (Santa Cruz Biotechnical, Santa Cruz, CA, USA) and ECL-plus (Amersham Biosciences, Little Chalfont, Bucks, UK) using chemiluminescence imaging (LAS 1000; Image Gauge v.3.0, Fuji, Tokyo, Japan).
PCR products were separated from free primers on a 5% native polyacrylamide gel, imaged on a Fuji FLA-5000 imandr, and quantified using Image Gauge software.
Quantification of bands was done by using a Bio-imaging analyzer LAS 1000 with Image Gauge Ver. 3.4X software (Fuji film).
Quantitation was carried out using image gauge software.
Quantification of western blots and autoradiographies was performed using Image Gauge V3.45 (Fuji, Tokyo, Japan).
Blots were visualised using a FLA3000 phosphoimager and Image Gauge software (Fujifilm Corp).
Cleaved PARP was quantified by Image gauge software and normalized by GAPDH expression (Figure 6B).
Differences in signal were quantified by densitometry using Image Gauge version 4.0 (Fujifilm).
Gels were dried onto 3 MM Whatman paper and analyzed by Phosphor Imaging using a Fuji FLA3000 and Image Gauge V3.3 software.
Proteins were detected using the enhanced chemiluminescence kit (Pierce) and bands quantified with Science Lab 2003 Image Gauge (Fujifilm, Tokyo, Japan).
