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Imaging fish carrying the Tg(fli1a EGFP y1 transgene (abbreviated here to fli1:eGFP) allows us to visualize possibly all cranial neural crest-derived mesenchyme (Figure 6).
For low-resolution images, fish were mounted in 3% Methyl-cellulose and observed with a stereomicroscope (Leica MZ10F) and images were taken with a CCD camera (Leica DFC 490).
Their siblings were kept as control; none of the imaged fish showed retarded development.
To allow a more detailed visualisation of the cell shapes, we imaged fish carrying the Tg(TDL358 GFP) transgene (labelling iridophores and glia with cytosolic GFP [ Levesque et al., 2013]) alone or together with a second transgene, Tg(sox10 mRFP), which labels neural crest derivatives with membrane-bound mRFP.
Artists brought the tools of big screen virtuosity, humor, beauty and sometimes brutality to images fished from the real world.
Indeed, the new image of fish has sparked all manner of creativity, with fish and shellfish starring on platters, napkin rings, glassware, jewelry, towels, clothing and even carpets.
President Zardari met me just inside the doorway, then seated himself facing a widescreen TV displaying an image of fish swimming in a deep blue sea.
Many of the fishermen, backed by environmentalists, say even the mere fact of drilling in the area could harm the pristine image their fish now enjoy.
Later, as things heated up, the space could shrink to almost zero, the figures crowding the image like fish in a too-full aquarium.
Indeed, the enveloping power of the past is captured in a startling image of fish washing up on a beach: "Out from the darkness of the sea, wave after wave of small, luminous bodies washed to shore".
The OCT cross-sectional image of fish cornea is acquired and shown in Fig. 6(a).
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