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An example of this method is voltage sensitive dye (VSD) imaging, which is often used to image changes in membrane potential [ 2].
The capability to image changes in cellular metabolism (if imaging FAD or NAD(P H) or the influx and conversion of 11- cis-retinol in cone inner segments in response to visual stimuli is of interest, not only in young and aging healthy eyes, but also in eyes with retinal pathology.
Positron emission tomography (PET) and single-photon emission computed tomography (SPECT) are non-invasive and radioisotope-based nuclear imaging techniques that can be used to image changes in tissue blood flow, which can provide early, sensitive, and specific detection of ischemic diseases at the molecular level [19, 20].
In a true zoom lens the image changes in scale but not in sharpness during zooming; some varifocal lenses, however, need refocusing at different focal lengths.
We note that the intensity of optical ring image changes in the edge.
From the image, changes in size and shape of the grains can be obviously observed.
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Figure 4(a), (b), and (c) show original image, change in the horizontal direction, and change in both horizontal and vertical direction respectively.
Until now there has been no diagnostic technique with sufficient spatial and temporal resolution to directly assess or longitudinally image change in lymphatic function and architecture with progressive disease in order to justify the addition of prophylactic treatments.
To further investigate the role of MCA in reperfusion, we directly imaged changes in blood flow in MCA's cortical branches using laser speckle imaging (LSI).
From AFM images, changes in membranes roughness were clearly observed.
We concurrently imaged changes in cAMP and Ca2+ by loading islets from transgenic mice with the calcium indicator dye Fura 2, and measuring ΔF470/F535 (for cAMP) and ΔF340/F380 (for Ca2+).
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