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This coupled setup allowed us to image cells with conventional wide-field deconvolution microscopy prior to super-resolution imaging in order to visualize Alexa 647-stained centrioles as reference to determine their orientation and age within a centriole pair whenever required.
However, introducing substrate surface textures often alters the ability to image cells with high precision, compromising our understanding of molecular mechanisms at stake in environmental sensing.
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Again, we imaged cells with two photon time lapse microscopy and followed it with post hoc immunohistochemistry (Fig. 5A E, E1).
(B) Zoom of the electron dense region shown in A. (C and D) Images of cells with electron dense regions located in the cytoplasm.
Imaging single cells with fluorescent markers over multiple cell cycles is a powerful tool for unraveling the mechanism and dynamics of the cell cycle.
(D ) Representative images of cells with one and two GFP dots are shown.
DNA FISH images, of cells with recorded RNA FISH signals, were acquired in the same way.
Images show cells with 1, 2, 3, 4, and 6 flagella (left to right).
This optical sectioning 3D reconstruction method of cell visualization yields high-quality images of cells with a cost-efficient approach with no special equipment needed prior to imaging.
To further document that SpoIIE constricts, we have taken additional time-lapse images of cells with the FtsZ ring and membrane labeled in addition to SpoIIE.
Ding and Eskelinen's criticism of our study is the lack of electron microscopy (EM) images from cells with inhibited autophagy.
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