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Images were collected using an AMT digital imaging system with proprietary image capture software (Advanced Microscopy Techniques).
We stained sections with toluidine blue (0.01% (Sigma-Aldrich), 0.01 M acetate, pH 4, filtered) for up to 10 minutes [50] and imaged them using a Retiga 1300 Camera with QImaging (image capture software suite 2.0), a Zeiss microscope with a Dell Optiplex GX240, and a resolution set at 1024×768.
The stained sections were viewed on a Hitachi H7500 transmission electron microscope and images were captured using an AMT XR-16 digital camera and AMT Image Capture software, v602.600.51.
Caliri used his own image capture software Dragonframe to make the video, which also comes in a 3D version on Nintendo's 3DS.
Several studies have measured nematode length or volume using microscope reticles or image capture software [25], [26].
Slides were then stained with hematoxylin and photographed using a Leica DM 4000B with Adobe Photoshop image capture software.
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The microchannel was measured to be 1,800 × 333 (599,400 pixels) via the image capturing software, and it corresponds to the total volume of one main channel in the viewing field: V A = L × w × d = 1,638 × 300 × 12 μm3 = 5,896,800 μm3.
Microscopic images were acquired with an Olympus BX51 light microscope/CCD camera set-up and ANAlysis image capturing software (Soft Imaging Systems GmbH, Münster, Germany).
Image Capturing software (DP Controller v. 1.1.1.65, 2002 Olympus) and Image Managing software (DP Manager v. 1.1.1.71, 2002 Olympus) were used to obtain digital microphotographs and to ensure accurate calibration of all measurements.
Image capturing software used in this study for the ImageJ method delayed this process by approximately 15 min, but was not used for the Image SXM method – a limitation of this study due to the lack of available equipment.
Microphotographs of cross-sections were taken at ×100 and ×400 magnifications using a digital camera (Scion Corporation, CFW-1310C, USA) attached to a light microscope (Olympus BX 50F, Olympus Co. Ltd ,Japan) and image capturing software Scion Visicapture, version 1.4 (Scion Corporation).
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