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Live-cell image and cell ultrastructure was observed.
It is difficult to validate or reject this assumption based on the SEM image of bagasse in Figure 1, since the orientation of the image and cell type featured may distort the apparent porosity [ 21].
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Figure 5 Confocal microscopy images and cell fluorescence intensity analysis.
Subsequently, cultures were imaged and cell number was determined every 24 h to monitor for reversal of morphology and proliferation.
We thank Guillaume Balavoine for support and the Imaging and Cell Biology facility of the IFR87 FR-W22511) for expert support with confocal microscopy.
Confocal images from CA1 region of hippocampus were obtained at a magnification of 40× using a Nikon Eclipse C1si Spectral Imaging Confocal Microscope System at the Nikon Imaging Center and Cell Imaging Facility at Northwestern University.
Using fractions instead of the direct object counts normalizes for the different number of cells in each image and different cell sizes.
Image analysis and cell counts were performed offline using SimplePCI (Hamamatsu).
Search results and images may be downloaded and direct visualization of each object/cell in the compendium is available through the companion Image Viewer and Cell Viewer.
A counting frame of 10.000 µm2 (Region of Interest; ROI) was superimposed on the calibrated images and cells were counted within this area using the image processing and analysis program ImageTool (UTHSCSA, USA) as described before [73] [75].
Further challenges in biological image processing are inherently low contrast images and cells changing their shape or momentum abruptly.
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