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To assess the content of particular molecules at synapses, confocal images were projected to create a two-dimensional image and analyzed with IPLab software (Scanalytics, Fairfax, VA, USA).
This strategy allows us to compare pixels from the SDR and non-SDR that have been measured under exactly the same conditions in a pairwise manner, since both measurements were derived from the same image and analyzed identically.
The gel was scanned with a PowerLook 2100XL from Techville, Inc. (Dallas, TX), converted to an 8-bit grayscale image and analyzed by Image J (NIH; http://rsb.info.nih.gov/ij/).
Following washing and scanning of the arrays, data was extracted from the scanned image and analyzed for normalized gene expression summary values (CALLS) by quantile normalization and the Robust Multi-array Average (RMA) algorithm [ 34] using the NimbleScan software (NimbleGen).
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These patterns can obscure the image and distract art conservators from reading the image and analyzing paint layers.
Different methods to image and analyze cdr2p intensities were compared.
We used deconvolution microscope to image and analyze the localization of Oct-4 mRNA.
The membranes were imaged and analyzed with the ChemiDoc MP imaging system (BioRad).
Cells were imaged and analyzed using a Zeiss LSM 510 Meta imaging system in the Massey Cancer Center Flow Cytometry and Imaging Facility.
At least three preparations for each experimental condition were imaged and analyzed.
Similar to Trichrome images, adjacent Herovichi stained sections were imaged and analyzed using a custom-written Matlab code.
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