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Transthoracic echocardiography was performed using a Toshiba ultrasonic image analyzing system (Nemio-XG) equipped with 7.5 MHz imaging transducer.
Both VEGF expression and newly formed bone were measured by a computer-assisted image analyzing system.
Newly formed bone was measured by a computer-assisted image analyzing system.
The bands were scanned and analyzed using the IPLabGel image analyzing system (Signal Analytics, Vienna, VA, USA).
An anionic fluorescent dye, LY, was injected in the same way and its concentration was estimated by a microscopic image analyzing system.
Semi-thin sections of 0.3µm were obtaining, colored in 1% toluidine blue, and evaluated with light microscope combined with image analyzing system.
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The spectral density of the bands was analyzed by Bandscan 5.0 image analyze system.
Histomorphometrical analysis was conducted by digitizing and examining slides with a ZEISS AXIOSCOPE microscope (magnifications of x5, x10, x25, and x40) equipped with a colour images analyzing system (software SAMBA IPS 4.27, SAMBA TECHNOLOGIES, France).
The density of each band was quantified using an image-analyzing system (i -MaxTM, CoreBio System Co., Seoul, Korea).
The density of each band was quantified using an image-analyzing system (i-Max™, CoreBio System Co., Seoul, Korea).
Images of PCR gels were recorded and the intensities of the PCR bands for both alleles were quantitated using an image-analyzing system (LabWorks 3.0, UVP, Cambridge, UK).
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