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Sections were analyzed with a fluorescence microscope (Zeiss) equipped with an imaging system and the image analyzing software Axio Vision.
Pre-/postoperative digital radiographs were downloaded into image analyzing software that calculated the amount of dentin removed.
Size distribution measurements for Au particles were performed on digital images by using the image analyzing software Image-Pro.
The fiber diameter of each specimen was measured by image analyzing software, Image J (National Institute of Health, USA).
Besides its sensors, the company has created image analyzing software that can zero in on just the vegetation, leaving out soil and shadows in post-processing analysis.
In order to develop a standard allometric equation, we measured leaf areas of the samples with an image scanner (Canon D2400U, Japan) and image analyzing software (Scion, Frederick, Md).
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Morphometric analysis was performed additionally for histologic quantitative evaluation using a digital camera (Nikon DXM 1200, Badhoevedo, Netherlands) combined with image-analyzing software (Lucia imaging software, Laboratory Imaging Inc., Prague, Czech Republic).
The VEGF positive cell area in five randomly selected fields was evaluated using NIH digital-image analyzing software, Image J 1.37v, (NIH, Bethesda, MD).
Leakage was quantified by linear measurement of dye penetration with the aid of image-analyzing software.
To quantitate tumor suppressive effects, the total area of tumors in each section stained with H&E was calculated using the image-analyzing software Winroof (Mitani Corporation, Tokyo, Japan).
The position of the center of gravity was calculated by image-analyzing software, which was used to calculate the total distance traveled by the mouse as well as the positional preference (either center or peripheral, where peripheral was defined as the area within 10 cm from the wall).
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