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To develop an image analysis device devoted to the measurement of donor corneal transparency (T), folding (F) and clear corneal diameter excluding gerontoxon (corneal arcus) (G) during organ culture.
The thickness of the calcium orthophosphate coatings was evaluated using an image analysis device.
The results were quantified through an automated image analysis device (SAMBA Technologies).
In the present immunohistochemical study of 924 invasive breast carcinomas, we investigated c Kit expression within tissue microarrays (TMA), quantified by densitometry on digitised microscopic images using an image analysis device and dedicated software.
Current histoprognostic criteria on H and E staining were not retained for statistical analysis, mainly to limit the burden of data and also to focus the statistical analysis on continuous variables homogeneously obtained by (numerical) densitometric measurement of immunoprecipitates with the image analysis device.
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Therefore, we recommend that experts review these published photographs and perform a complete ophthalmological examination based on stereophotographs, RNFL photographs and analysis with digital image analysis devices.
However, appropriate software dedicated to immunocytochemical tissue labelling, along with the latest image analysis devices, can considerably facilitate the rapid, high-throughput measurement of immunostaining in hundreds of immunostained cores within TMA tissue sections, with completely automated digitisation of microscopic slides (up to 50 slides per batch) in only a few hours.
Scanning was performed using a GenePix Personal 4100A scanner (Molecular Devices Corporation, Sunnyvale, CA, USA), and data were analyzed using GenePix Pro 6 Microarray Image Analysis (Molecular Devices Corporation).
Images were acquired on a high content screening microscope (ImageXpress Micro, Molecular Devices), and image analysis (MetaXpress3.0, Molecular Devices) was performed to obtain an average H3K79me2 signal per cell.
A secondary objective was to evaluate the potential use of quantitative image analysis with this device for objective classification of abnormal sites.
We used the GenePix 4000A optical scanner (Molecular Devices, Sunnyvale, CA, USA) and the GenePixPro 5.1 image analysis software (Molecular Devices) to quantify the gene expression level intensities.
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