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Image acquisition throughput could be increased further by adapting and/or reducing the number of imaging time points (as done in this study shortening 0…2 h dynamic to 1 2 h static scans) and by increasing the number of animals in the field of view (e.g. with a 3D-printed 4-bed hotel (inviCRO)).
Hence, their image acquisition throughput is limited by that of CCD and CMOS cameras.
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In the current study, high-throughput image acquisition and analysis was used to study the salinity tolerance traits of two rice cultivars (IR64 and Fatmawati) under different levels of salt stress.
This instrument is commonly used to perform high-throughput image acquisition of cultured cells living in 96- or 384-well plates.
The Ikoniscope robotic, high-throughput, image acquisition and display microscopy system, is developed by Ikonisys for rare cell identification and analysis.
Contact: [email protected] The use of high-throughput image acquisition, such as in phenotypic screens, has been quickly increasing and thus provides a new source of data for computational biologists.
However, developing in toto imaging technologies for acquiring large amounts of data will necessitate improving the speed and throughput of microscopic image acquisition and analysis.
Besides the possibility of high throughput image acquisition, this enabled us to leave acquisition parameters unchanged by excluding software or hardware upgrades in order to ensure data stability and comparability over time.
Besides the possibility of high throughput image acquisition, this enabled us to maintain acquisition parameters unchanged by excluding software or hardware upgrades in order to ensure data stability and comparability over time.
Since total assay throughput is limited by image acquisition speed, we utilized a derivation of Devore [27] to estimate the minimum number of cell measurements needed per well to achieve significance (Methods).
Automated image acquisition was performed with an ImageXpress high-throughput screening system (Molecular Devices) using a ×10 objective.
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