We confirmed that this extended conformation was not due to rapid motions of GFP-ParB/ GFP-ParB/ng image acquisition as it was readily observed in formaldehyde-fixed cells.
In contrast with current applications used to determine MD from digital mammograms, which have to be present as the image is being acquired by the machine, ImageJ can be applied at any time after image acquisition, making it feasible to read digital images retrospectively.
In addition to MR thermometry, MR can now be used for the guidance of the tumour targeting with 'fluoroscopic' rapid image acquisition, and it can provide several contrast mechanisms for post-procedural assessment of the extent of the thermal coagulation zone.
Among image acquisition improvements, it is possible to mention free multivoxel angulation, a better adjustment of the field of view to the mass or nodule under analysis and a better signal-to-noise ratio, in addition to the two-dimensional sequence adopted.
However, recent developments in image acquisition have made it possible to segment the hippocampus into its subfields in a fully automated fashion and this method has now been validated using ultra high resolution MRI (Van Leemput et al. 2009).
However, the external plastic sheet often appears to be deformed during vessel flushing, image acquisition and when it is pushed against the vessel wall and can thus not be used for robust calibration.
In contrast to some of the other methods (for example, Shepherd et al, 2011), it does not require the use of any special equipment (for example, phantoms, step wedges) during image acquisition and therefore it can be applied to historical collections of images.
Although the sample suffered irradiation damage, image acquisition was performed on it at ambient temperature for convenience.
By varying the image acquisition angle in the simulations, it is possible to move the laser scanning direction from the x-axis (angle 0°) to the y-axis (90°), as explained in the protocol for the experiments (Fig. 1).
The ease of adding DCE-CT into standard PET/CT acquisition protocols has been demonstrated[, 21 23] and CTTA data are post-processed, thus extra image acquisition is not required and it can be applied to both pre-acquired PET/CT and DCE-CT data.
