We wrote dedicated software in Matlab 7.2 to control every step of the experiment using a graphical user interface : image acquisition (using Matlab Image Acquisition Toolbox 2.10), microscope driving, temperature, pump and electrovalves control.
Further development of a real-time image acquisition and analysis system interfaced to the fluorescence endoscope will allow acquired images to be analysed on the spot within automatic or user-selected regions of interest to extract image parameters.
The ESRF readout system is based on a custom interface board named Parallel Readout Image Acquisition for Medipix (PRIAM) a fast PCI interface and a Linux PC.
The Camera Link frame-grabber (FlexRIO Solution from National Instruments) includes a field programmable gate array (FPGA) for image acquisition using a Camera Link interface; the FPGA allows for the codification of ad-hoc image processing algorithms using LabVIEW/FPGA software.
Images were recorded with a CCD camera (Photometrics CoolSNAP HQ2) interfaced to image acquisition software (Compix SimplePCI).
Control and image acquisition are performed via an intuitive computer interface.
The schematic diagram in Figure 1 shows the setup of a fluorescence endoscopy system (Karl Storz Endoscope, Karl Storz GmbH & Co., Tuttlingen, Germany) interfaced to a PC-based image acquisition and analysis system for fluorescence diagnostic imaging of human oral lesions in the clinic.
Images were acquired every 2 s for 30 min on a fluorescent microscope using MATLAB interfaced with Micromanager Software for image acquisition and automated valve control.
In the application's main interface, the button labeled as "Image Acquisition" activates the camera.
In the paper, special attention is devoted to the following knowledge-based system components: system-core components (the database, the knowledge base, the inference engine, and the user interface) and application-specific components (image acquisition, the resolution pyramid, image pre-processing and feature extraction, image registration, object segmentation, fusion, and data analysis).
