Previously published studies using ImageJ method report using a microscope with a x100 objective, while the Image SXM method requires a x40 objective, both with digital image acquisition capabilities.
SPIM is an attractive technique for in vivo imaging due to its widefield image acquisition, inherent depth sectioning capability and low sample exposure to laser excitation light [ 4].
Lanczos 3 and B-spline interpolation are comparable to rotating the textures prior to image acquisition, whereas the recognition capability is significantly and increasingly lower for the frequently used third order cubic, linear and nearest neighbour interpolation.
Compared to classical sensors performing motion detection downstream the image acquisition, the offered processing capabilities are somehow limited, but the chosen analog architecture, on which they are implemented, offers a better compromise between power consumption and algorithm performance.
The good spatial resolution, high contrast resolution, fast image acquisition and multi-planar reconstruction capabilities of computed tomography make this imaging modality very attractive for imaging the bowel as well.
To integrate an optical tomography approach in a SPIM microscope we took advantage of three features of our existing SPIM setup: (1) fast image acquisition with high frame rate sCMOS cameras; (2) multiview capability, i.e. the sample can be quickly rotated; and (3) LED for back illumination, which can provide transmission images of the specimen.
In this work we show that new technological advances on automated dispersion of granular materials imaged with a camera-coupled microscope and enhanced computer capabilities enable fast and high resolution image acquisition of thousands of ash grains that resolve this limitation.
Standardised and formal training in image acquisition and image interpretation are vital to optimize the diagnostic capabilities of this imaging technique, and indeed, technical developments continue to be made.
HDR was characterized till 83.8 dB, limited only by the characterization setup and the chip specific configuration, capability for higher dynamic range has been demonstrated by image acquisition and processing.
Image acquisition was done with Volocity or LSM imaging software.
