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The illumination was set to a constant value of 1100 μmol m−2s−1 using an external halogen lamp to ensure the light-saturation of the photosynthesis.
The background light illumination was set using a LOT Oriel LS0106 solar simulator with an AM 1.5-G filter, and light intensity was adjusted using appropriate neutral density filters; a 532-nm CryLaS (Berlin, Germany) FTSS 355 50 laser at a frequency of 18 Hz with an intensity of approximately 7 mW cm−2 was used to cause the small perturbations (1-ns pulse width) in the cells.
Illumination was set to 150 lx.
The illumination was set at 400 lx.
During the examination, background illumination was set at 1.27 cd/m.
Twelve-micrometer-thick optical sections were acquired at 4 μm steps and YFP illumination was set to 100 ms and 50%% neutral density (ND) filter, mCherry illumination was set to 150 ms and 50 % ND.
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PPFD and duration of actinic illumination were set to 1300 μmol photons m−2 s−1 and 3 s, respectively.
Once the illumination is set, the multiple scattering and the inhomogeneity of the studied region control the characteristics of the diffuse field.
But when the headlights are on at night, the background illumination turns red orange and it is hard to see the letters on the clock and radio, even when the illumination is set to maximum brightness.
The phase contrast annulus in the condenser is also swung out of position so that the illumination is set for bright-field measurements.
If an invalid sequence has been selected, no illumination is set and the user must start again (error path in Fig. 4).
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